Journal: The American Journal of Pathology

Article Title: Hematopoietic Fas Deficiency Does Not Affect Experimental Atherosclerotic Lesion Formation despite Inducing a Proatherogenic State

doi: 10.1016/j.ajpath.2011.02.011

Figure Lengend Snippet: Hematopoietic Fas−/−→Ldlr−/− chimeric mice do not show changes in atherosclerotic lesion area, cellularity, or apoptosis. WT→Ldlr−/− (n = 15) and Fas−/−→Ldlr−/− mice (n = 11) were generated and fed with an 0.5% cholesterol diet for 16 weeks. A: Flow cytometry was performed on peripheral blood of the bone-marrow chimeric mice at the time of sacrifice. Representative profiles are shown. Green indicates background staining with no antibody in Fas−/−→Ldlr−/− mice; red indicates positive staining with CD95 antibody in WT→Ldlr−/− chimeric mice; and blue indicates lack of staining with CD95 (Fas) antibody in Fas−/−→Ldlr−/− chimeric mice. B: Atherosclerotic lesion area was determined from H&E-stained brachiocephalic artery sections (6 per mouse, 50-μm intervals) using ImageJ software. DAPI-positive cells (C) and TUNEL-positive cells (D) were counted from brachiocephalic artery sections (6 to 10 sections per mouse), using fluorescence microscopy. Each symbol represents an individual mouse; horizontal lines indicate the mean value. P > 0.05, Mann-Whitney U-test (B, C, and D).

Article Snippet: Plasma cholesterol levels were measured at the Northwest Lipid Research Laboratories (Seattle, WA).

Techniques: Generated, Flow Cytometry, Staining, Software, TUNEL Assay, Fluorescence, Microscopy, MANN-WHITNEY

Journal: The American Journal of Pathology

Article Title: Hematopoietic Fas Deficiency Does Not Affect Experimental Atherosclerotic Lesion Formation despite Inducing a Proatherogenic State

doi: 10.1016/j.ajpath.2011.02.011

Figure Lengend Snippet: Hypercholesterolemia is increased in hematopoietic Fas−/−→Ldlr−/− chimeric mice. WT→Ldlr−/− (n = 15) and Fas−/−→Ldlr−/− mice (n = 11) were generated and fed with an 0.5% cholesterol diet for 16 weeks. A: Total plasma cholesterol levels. *P < 0.001, Student's t-test. Data are reported as means ± SD. B: Fast protein liquid chromatography profiles were performed on pooled plasma samples from WT→Ldlr−/− and Fas−/−→Ldlr−/− mice.

Article Snippet: Plasma cholesterol levels were measured at the Northwest Lipid Research Laboratories (Seattle, WA).

Techniques: Generated, Clinical Proteomics, Fast Protein Liquid Chromatography

Journal: The American Journal of Pathology

Article Title: Hematopoietic Fas Deficiency Does Not Affect Experimental Atherosclerotic Lesion Formation despite Inducing a Proatherogenic State

doi: 10.1016/j.ajpath.2011.02.011

Figure Lengend Snippet: Microvascular inflammation is observed in hematopoietic Fas−/−→Ldlr−/− chimeric mice. Liver and lung tissue from Fas−/− and WT hematopoietic chimeras generated on Ldlr−/− background were evaluated after 16 weeks on an 0.5% cholesterol diet. Adjacent sections were stained with H&E, the macrophage antibody Mac-2, the B lymphocyte antibody B220, or the T lymphocyte antibody CD3. Original micrographs, ×20.

Article Snippet: Plasma cholesterol levels were measured at the Northwest Lipid Research Laboratories (Seattle, WA).

Techniques: Generated, Staining

Journal: The American Journal of Pathology

Article Title: Hematopoietic Fas Deficiency Does Not Affect Experimental Atherosclerotic Lesion Formation despite Inducing a Proatherogenic State

doi: 10.1016/j.ajpath.2011.02.011

Figure Lengend Snippet: Comparison of Global and Hematopoietic Fas-Deficient and FasL-Deficient Models and Their Effect in Mouse Models of Atherosclerosis

Article Snippet: Plasma cholesterol levels were measured at the Northwest Lipid Research Laboratories (Seattle, WA).

Techniques: Comparison, Western Blot, Control